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Lactobacillus
sporogenes
- vedi:
Batteri autoctoni
+
Microbi
Erroneamente identificato nel 1932 - Horowitz -
Wlassowa e da Nowotelnow ed inizialmente accettato come Lattobacillo nel Bergeys,
manual 5° edizione (1935) è stato poi successivamente Riclassificato nel genus bacillus
nelle edizioni del manuale successive alla 6° edizione (si veda ad esempio a pag. 577
della 8° Edizione del Bergeys del 1974).
Successivamente
e stato anche riconosciuto che il bacillus sporogenes era identico al bacillus
coagulans e che quindi non costituiva specie a sè stante e pertanto non esisteva (si veda ad esempio lo Stedmans Bergeys
Bacterìa words 1992).
In
effetti la g.r.a.s. list del F.D.A. americana del 1989 riporta solo il Bacillus Coagulans
ed ignora totalmente il bacillus Sporogenes.
Concludendo:
il lattobacillo sporogenes non è un lattobacillo ma un bacillo (un genus
diverso) e la specie Sporogenes non ESISTE essendo identica al Coagulans. Scientificamente quindi si sono prese lucciole per lanterne
e commercialmente pare strano; ai giorni nostri sarebbe come vendere riso
chiamandolo avena.
In
realtà dovrebbe essere Clostridium Sporogene, parente stretto del
Botulinum, ma
senza la tossina
(vedi bibliografia)
Quanto
alla sua efficacia come probiotico non esistono studi specifici su riviste scientifiche
indipendenti e di prestigio Internazionale (British Medical Journal,
Bacteriological
Revieus, the American Journal of Clinical
Nutrition e simile livello), ma solo letteratura sponsorizzata dal produttore indiano su riviste indiane ed estremo orientali (coreane in
particolare).
Come
commento sulla eventuale efficacia probiotica del prodotto si può dire che il Bacillus
Coagulans (il suo vero nome), come tutti i batteri non fa parte della
flora batterica dominante dellintestino umano (la flora
autoctona formante Colonie, stabilmente presente, ad azione probiotica certa) ne' di quella sottodominante (autoctona) bensì di quella
fluttuante
(alloctona, opportunista e transiente e quindi non stabilmente presente nellintestino
umano) che nel suo insieme non rappresenta più del 0.02% del totale compredendovi anche
tutti gli altri batteri del gruppo, i lieviti e i micromiceti.
Va
detto inoltre che i bacilli sporogeni (ad esempio bacillus sphaericus, bacillus
subtilis)
vanno visti con estrema cautela in quanto sono stati implicati spesso in avvelenamenti alimentari.
vedi:
Falsita'
della medicina
ufficiale +
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MINISTERO SALUTE
italiano informato sui Danni dei Vaccini
+
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(Mineral-Vitaminici) e Farmaci a CONFRONTO
+ Pericolo
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+
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Generally growing at 5.0
or Iess; at neutral or initial alkaline reactions lag phase may be Iengthened or total
growth yield reduced.
Found
in dairy products and effluents, grain and meat products, water, sawage,
beer, wine,
fruits and fruit juices, pickled vegetables, sourdough and mash; also parasitic in the
mouth, intestinal tract and vagina of
many homothermic animals including man. Pathogenicity ia highly unusual.
The
G + C content of the DNA ranges from 34.7 # 1.4 - 53.4 # 0.5 moles %
(buoyant
density). Type species: Lactobacillus delbrueckii (Leichmann) Beijerinck 1901, 229.
Opin. 38, Jud. Comm. 1971, 104.
Further Comments
The following, included in The Manual 7th ed., are here excluded from
Lactobacillus: la
caucasicus Beijerinck 1901, the previoue type species, is a rejected name (Opin. 38,
Jud. Comm. 1971, 104).
Lactobacillus bifidus is in transferred to Bifidobacterium
3.
Strains whose descriptions
are consistent with the original description of L. thermophilus Ayers and
Johson 1924, 291 produce endespores, catalase and cytochromes and are probably very
similar, if not identical, to Bacillus coagulans (Sharpe, 1962; Kitahara and
Suzuki, 1963).
4.
L. Pastorianus
(van Laer) Bergey et 1923 (see Speces incertaesedis).
Various
spore-bearing rods which produce lactic acid, aro facultative or aerobic and catalase
positive, have generally correctly been assigned to the genus Bacillus. Among these are: Baccillus coagulans 1915, 129; Bacillus circulans Jordan 1890, 831; bacillus lactis-termophilus (sic)
Gorini 1894, 16
(not validly pubIished - Rule 14a (1)); Bacillus dextrolacticus Berry 1933, 72;
Bacillus
dextrolacticus Werkman and Andersen 1940, 187;
Lactobacillus sporogenes Horowitz-Wlassowa and Nowotelnow 1932, 333.
Other
npore-forming rods which produce lactic acid homofermentatively, aro catalase negative,
not strict. anaerobes and otherwise closely resemble Iactobacilli have been placed in the genus Sporolactobacillus (p.
550). Organisms similar to
Sporolactobacillus
isolated from the rhizosphere of various wild pIants are Bacillus, Laevolacticus
Nakayama and Yanoshi 1967, 149 md Bacillus racemilacticus Nakayama and Yanoshi 1967, 149.
Unnamed
catalese negative rods from chicken meat, presumably related to both Iactobacilli and
aerobic sporeformers, were isolatied by Thornley and Sharpe (1959). Glucose was converted homofermentatively to Iactic
acid but growth did not occur in media favoring the growth of
Iactobacilli, spores were not observed and some strains had coryneform
morphology.
The
micro-organisms of the San Francisco sourdough bread process probably include
heterofermentative Iactobacilli having a high CO2, and maltose requirement, in apparent inability to ferment any otber carbohydrate
and a need for fatty acids ma satisfied by Tween 80. Kline md Sugihara (1971) did not name the
organism.
Cillobacterium
cellulosolven Bryant et al. 1958, 533 praduces >90% lactic acid from
cellobiose.
It
has been suggested that the organism be included in Lactobacillus. Howewer, similar
rumen strains also produce butyrate and valerate and van Gylswyk and Hoffman (1970) suggest broadeing the Bryant et aI. (1958)
definition of Cillobacterium to include these characteristics. It would, of
course, be odd to include cellulose - dìgesting bacteria in
Lactobacillus.
Strictly
anaerobic Lactobacillus strains are known. Those from the rumen resemble or are L. Iactis
(Mann and Oxford, 1954; Jensen et al., 1956), L. Leichmannii or similar
organisms. In addition, however,
some anaerobic strains now assigned to other genera probably are Iactobacilli and such organisms as Eubacterium
crispatum, Brygoo and Aladame 1953.
641; Eubacterium- disciformans (Massini) Prévot 1938, 295; and Catenabacterium catenaforme
(Eggerth) Prévot 1938, 296,
are here provisionally Iisted under Species incertae sedis.
Key
to the apeclea of genus Lactobacillus
I.
Homofermentative.
Lactic acid is the major product from glucose (generilly 85% or more).
A. No gas from glucose or gluconate; ribose not fermented thiamine not
required; aIdolase activity;
D- or L- or DL-Iactid acid produced; G + O = 34.7-50.8%; generally grows at 45 C or
higher, generalIy not at 20 C and not at 15 C.
Colonies normally rough becoming smooth and compact in the presence of Tween 80 or
sodium oleate (Rogosa and Mitchell, 1950).
1.
Produce D()-Iactic acid.
1.
L. delbrueckii L. leichmannii
L.jenscnii meat broth with stringy aediment; better growth in glucoee
broth.
Ferment.ation
producta from peptone, yeast extract medium include acetic, isobutyric, isovaleric acids
and smaller amounts of propionic and isocaproie acids. Milk
is digested. Optimum temperature for growth is 30-37 C. Has
been Found in soil. Reference strain: ATCC 25761; NCIB 10639.
19.
Clostridium sporogenes (Heller) Bergey et al.
1923,329 var. A Metchnikoff 1908, 944; Metchnikovillus sporogene.S Heller 1922, 29;
Closlrùlium sporogenes var A (Metchnikoff) Prévot 1938, 83.) Spo.ro
ge.nes. M.L. n. spora a spore;
Gr. v. gennaio produce; M.L. part.adj. sporogenes
sporeproducing. Straight rods, 0.30.4 by 1.46.6
pm. Motile with peritrichous flagella. Spores are oval, subterminal.
Gram-positive.
Cell waIl contains DL.- diaminopimelic acid; cell wall sugar is galactose.
Surface
colonies on most solid media, 2-6 mm in diameter, raised whitish to yellowish center with
gray rhizoids, Medusa head margin, semiopaque, matt surface. On moist media, colonies are flat, thin,
spreading.
Abundant growth in nutrient, cooked meat broth; grayish yellow sediment.
Acid
production from carbohydrates often masked by ammonia from amino acid
deamination.
Fermentation products include large § amounts of butyric acid with smaller amounts of acetic, isobutyric, isovaleric and
isocaproic acids and propyl, isobutyl and isoamyl alcohols. MiIk
is digested.
Optimum
temperature for growth is 30- 40 C; grows at 25- 45 C. Has
been found in soil, wounds, food, intestinal contents. The
G + C content of the DNA in 26 moles %.
Reference strain: ATCC 3584; NCIB 10696.
20. Clostridium botulinum (Van Ermengem)
Bergey et al. 1923, 328
Bacillus botulinus van Ermengem 1896, 443; Ermenemi1Lus bottdinus (van
Ermengem) Helier
1922
28; Botulobacillus botulinus (van Ermengem) Orla-Jensen 1909, 343.) bo.tu.li
num. L.
n. botulus sausage;
M.L. adj. botulinum pertaining to sausage.
Six
toxin types, A, B, C, D, E, F, G, are differentiated on the serological specificity of
the toxin, which is not necessarily correlated with the cultural properties of the organism.
Type
A and Proteolytic Strains of Types B. C, D, and F. Straight to slightly curved rods 0.8 -
1.3 by 4.4 - 8.6 pm. Motile with peritrichous flagella. Spores are oval, subterminal with no exosporium. Gram- positive. Celi
wall contains DL- diaminopimelic acid; cell wall sugar is glucose.
Colonies
are circular, 3 - 8 mm in diameter, opaque center, rhizoid margin.
translucent, gray, matt
to semi-glossy surface.
Abundant growth in nutrient broth, cooked meat broth, with uniform
turbidity,
grayish sediment.
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